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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
Biophysical studies on chitosan-coated liposomes
Mohsen M Mady1, Mirhane M Darwish, Safaa Khalil
1Biophysics Department, Faculty of Science, Cairo University, Giza 12613, Egypt. dr_mmady@yahoo.com
European Biophysics Journal : EBJ
|August 4, 2009
Summary
Chitosan coating enhances dipalmitoyl phosphatidylcholine (DPPC) liposomes, increasing their size and membrane resistance. This combination improves liposomes for drug delivery applications.
Area of Science:
- Materials Science
- Biotechnology
- Pharmaceutical Sciences
Background:
- Liposomes are versatile drug delivery vehicles, aiding drug stabilization and targeted delivery.
- Chitosan, a biopolymer from crustacean shells, offers potential in drug development and tissue engineering.
Purpose of the Study:
- To investigate the interaction between chitosan and dipalmitoyl phosphatidylcholine (DPPC) liposomes.
- To evaluate how chitosan coating affects liposome properties relevant to drug delivery.
Main Methods:
- Transmission electron microscopy (TEM) for visualizing liposome structure.
- Zeta potential measurements to assess surface charge.
- Solubilization studies with octylglucoside (OG) to determine membrane integrity.
- Fourier transform infrared (FTIR) spectroscopy and viscosity measurements to analyze chemical interactions.
Main Results:
- Chitosan coating was confirmed by TEM and increased liposome size by approximately 92 nm.
- Zeta potential became more positive with increasing chitosan concentration, indicating successful surface modification.
- Chitosan-coated liposomes exhibited increased resistance to detergent solubilization, suggesting altered membrane properties.
- FTIR analysis revealed changes in DPPC spectral bands upon chitosan interaction.
Conclusions:
- Chitosan coating modifies DPPC liposomes, enhancing their size and membrane stability.
- The altered properties suggest potential for improved drug delivery efficacy.
- Combining liposomal and chitosan characteristics offers a promising strategy for advanced drug delivery systems.
