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Related Concept Videos

Phosphodiester Linkages01:01

Phosphodiester Linkages

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Cytoskeletal Linker Proteins - Plakins01:09

Cytoskeletal Linker Proteins - Plakins

Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...

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Related Experiment Video

Updated: Jun 21, 2026

Experimental Approaches for the Synthesis of Low-Valent Metal-Organic Frameworks from Multitopic Phosphine Linkers
07:14

Experimental Approaches for the Synthesis of Low-Valent Metal-Organic Frameworks from Multitopic Phosphine Linkers

Published on: May 12, 2023

Recent advances in traceless linkers.

A B Reitz1

  • 1The RW Johnson Pharmaceutical Research Institute, Drug Discovery Division, Spring House, PA 19477, USA. areitz@prius.jnj.com

Current Opinion in Drug Discovery & Development
|August 4, 2009
PubMed
Summary
This summary is machine-generated.

Traceless linkers in solid-phase synthesis enable cleaner small molecule library preparation. These advanced linkers attach and detach without leaving a trace, simplifying purification and improving target molecule integrity.

Related Experiment Videos

Last Updated: Jun 21, 2026

Experimental Approaches for the Synthesis of Low-Valent Metal-Organic Frameworks from Multitopic Phosphine Linkers
07:14

Experimental Approaches for the Synthesis of Low-Valent Metal-Organic Frameworks from Multitopic Phosphine Linkers

Published on: May 12, 2023

Area of Science:

  • Organic Chemistry
  • Synthetic Chemistry
  • Medicinal Chemistry

Background:

  • Solid-phase organic synthesis is crucial for creating diverse small molecule libraries.
  • Traditional solid-phase methods can leave detectable byproducts or require complex purification.
  • The development of novel linker technologies is essential for advancing synthetic efficiency.

Purpose of the Study:

  • To review recent advancements in traceless linkers for solid-phase organic synthesis.
  • To highlight the benefits of traceless linkers in small molecule library preparation.
  • To discuss the impact of traceless linkers on synthetic methodologies.

Main Methods:

  • Review of literature on traceless linkers in solid-phase synthesis.
  • Analysis of reaction mechanisms and outcomes involving traceless linkers.
  • Summarization of key examples and applications.

Main Results:

  • Traceless linkers facilitate the synthesis of small molecules where the attachment point is replaced by hydrogen.
  • These linkers simplify purification by eliminating the need to remove linker-derived byproducts.
  • Recent advances have expanded the scope and utility of traceless linkers.

Conclusions:

  • Traceless linkers represent a significant improvement in solid-phase organic synthesis.
  • Their application leads to cleaner products and more efficient library generation.
  • Continued development of traceless linker technology will further enhance synthetic capabilities.