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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Induced pluripotent stem cells: will they be safe?

Mathilde Jalving¹, Hein Schepers

¹John Radcliffe Hospital, Weatherall Institute of Molecular Medicine, Oxford, UK. hildejalving@googlemail.com

Current Opinion in Molecular Therapeutics

|August 4, 2009

Summary

Induced pluripotent stem (iPS) cells offer regenerative medicine potential but carry safety risks. This review details iPS cell generation and addresses critical safety concerns for therapeutic applications.

Area of Science:

Stem Cell Biology
Regenerative Medicine
Cellular Reprogramming

Background:

Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells using external factors.
iPS cells hold potential for various applications, including disease modeling and regenerative therapies.
Significant safety concerns impede the clinical translation of iPS cell technology.

Purpose of the Study:

To review the techniques for generating iPS cells.
To discuss the identified safety concerns associated with iPS cell technology.
To explore current strategies for addressing these safety issues.

Main Methods:

Review of current literature on iPS cell generation.
Analysis of safety risks related to reprogramming factors and cell differentiation.

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Discussion of methods to mitigate identified risks.

Main Results:

iPS cell generation techniques are established.
Key safety concerns include factor delivery, cellular alterations, and incomplete differentiation.
Ongoing research focuses on improving safety and characterization.

Conclusions:

iPS cells present a promising avenue for regenerative medicine and disease modeling.
Addressing safety concerns is paramount for the clinical application of iPS cell therapies.
Continued research is essential to ensure the safe and effective use of iPS cells.