Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Utility of Rapid Whole-Genome Sequencing in Hospitalized Adults With Unexplained Neurologic Presentations.

Neurology·2026
Same author

Development of a large porcine model of osteogenesis imperfecta type I.

Bone reports·2026
Same author

Vascular Tumors Result from Adeno-Associated Virus-9 Angiogenic Gene Therapy of Bone Allografts.

Vascular cell·2025
Same author

Semiautomated approach focused on new genomic information results in time and effort-efficient reannotation of negative exome data.

Human genetics·2024
Same author

Thoracic Aortic Aneurysmal Disease: Comprehensive Recommendations for the Primary Care Physician.

Mayo Clinic proceedings·2024
Same author

Impact of Genetic Disorders in the Surgical Management of Congenital Heart Disease.

World journal for pediatric & congenital heart surgery·2023

Related Experiment Video

Updated: Jun 21, 2026

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
06:41

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors

Published on: October 20, 2023

Adeno-associated virus vector integration.

David R Deyle1, David W Russell

  • 1University of Washington, Department of Biochemistry, Seattle, WA 98195, USA.

Current Opinion in Molecular Therapeutics
|August 4, 2009
PubMed
Summary
This summary is machine-generated.

Adeno-associated virus (AAV) vectors can integrate into host DNA, potentially causing insertional mutagenesis. This integration, while sometimes beneficial for gene therapy, has been linked to tumor development in mice, raising clinical safety concerns.

More Related Videos

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
09:12

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Published on: April 5, 2024

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
07:21

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Published on: June 30, 2021

Related Experiment Videos

Last Updated: Jun 21, 2026

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
06:41

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors

Published on: October 20, 2023

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
09:12

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Published on: April 5, 2024

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
07:21

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Published on: June 30, 2021

Area of Science:

  • Gene therapy
  • Molecular biology
  • Virology

Background:

  • Adeno-associated virus (AAV) vectors are widely used for gene delivery due to efficient transduction and long-term transgene expression.
  • AAV vector genomes typically persist as episomes but can integrate into the host genome.
  • Integration can occur via non-homologous end joining or homologous recombination.

Purpose of the Study:

  • To investigate the implications of AAV vector integration in vivo.
  • To assess the potential risks associated with AAV vector integration, particularly insertional mutagenesis.

Main Methods:

  • Analysis of AAV vector integration sites in experimental models.
  • Evaluation of insertional mutagenesis and its consequences.

Main Results:

  • AAV vector integration was observed in various experimental settings.
  • Insertional mutagenesis resulting from AAV vector integration was linked to tumorigenesis in mice.
  • Integration can be essential for therapeutic efficacy in some cases.

Conclusions:

  • While AAV vectors are promising for gene therapy, their integration into the host genome presents potential risks.
  • Insertional mutagenesis and subsequent tumorigenesis are critical safety considerations for clinical applications of AAV vectors.