Related Experiment Video
Updated: Jun 21, 2026
07:04
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
Cytotoxicity of resin-based light-cured liners
Josimeri Hebling1, Fernanda Campos Rosetti Lessa, Indri Nogueira
1Department of Orthodontics and Pediatrics Dentistry, School of Dentistry, University of São Paulo State, UNESP, Araraquara, Brazil.
American Journal of Dentistry
|August 5, 2009
Summary
Resin-based liners TheraCal (TCMTA), Vitrebond (VIT), and Ultrablend Plus (UBP) exhibit varying cytotoxic effects on pulp cells. VIT and UBP showed significant cell damage and reduced protein expression compared to TCMTA.
Area of Science:
- Biomaterials Science
- Dental Materials
- Cell Biology
Background:
- Resin-based dental materials are widely used as liners.
- Understanding their cytotoxicity is crucial for pulp protection.
Purpose of the Study:
- To evaluate the cytotoxic effects of three light-cured resin-based liners on cultured pulp cells.
Main Methods:
- Extracts from TheraCal (TCMTA), Vitrebond (VIT), and Ultrablend Plus (UBP) were prepared after 24 hours and 7 days.
- Pulp cells were exposed to extracts, and cytotoxicity was assessed via MTT assay (metabolism), total protein analysis, and SEM (morphology).
Main Results:
- All liners reduced cell metabolism and protein expression, with VIT and UBP showing greater effects than TCMTA.
- Cellular morphology was significantly altered by VIT and UBP extracts, unlike TCMTA.
- A reduction in cell number was observed for all tested liners.
Conclusions:
- TheraCal (TCMTA) demonstrated less cytotoxicity compared to Vitrebond (VIT) and Ultrablend Plus (UBP).
- VIT and UBP may pose a higher risk of pulp cell damage due to significant morphological alterations and reduced protein expression.
