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Related Concept Videos

Local Anesthetics: Differential Sensitivity of Nerve Fibers01:24

Local Anesthetics: Differential Sensitivity of Nerve Fibers

Local anesthetics (LAs) block the sodium channels of nerve trunks, sensory nerve endings, and neuromuscular junctions. Although LAs can block all kinds of nerves, the sensitivity of nerve fibers differs according to nerve types and structures. LAs are known to block myelinated fibers faster than unmyelinated ones. Also, they block pain or sensory neurons at low concentrations without affecting the motor neurons involved in muscle contractions. This helps relieve labor pain without affecting the...
Desensitization and Tachyphylaxis01:20

Desensitization and Tachyphylaxis

Tachyphylaxis is described as a rapid decrease in response to a drug after repeated or continuous administration of the same drug dose. It is a phenomenon where the body becomes less responsive to a particular substance or intervention over time, requiring higher doses or stronger interventions to achieve the same effect. It results from adaptive changes in the body's receptors, signaling pathways, or physiological processes that occur in response to prolonged exposure to a stimulus.
Several...
Local Anesthetics: Mechanism of Action01:23

Local Anesthetics: Mechanism of Action

Local anesthetics (LAs) block sensory and motor impulses by inhibiting the sodium channels on the nerve cell membranes. This induces temporary loss of sensation, relieving pain in a specific body area.
Local anesthetics are amphiphilic molecules consisting of a hydrophobic aromatic part linked to a hydrophilic group by an ester or amide linkage. They are weak bases and are usually available as salts, which increases their solubility and stability. Once administered, LAs exist in the body either...
Masking and Demasking Agents01:19

Masking and Demasking Agents

EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
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GPCR Desensitization01:12

GPCR Desensitization

G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
Permeability of Concrete01:25

Permeability of Concrete

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Related Experiment Video

Updated: Jun 21, 2026

Inducing Apical Periodontitis in Mice
10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

Effect of desensitizing agents on dentin permeability.

Hiroshi Ishihata1, Masafumi Kanehira, Tomoko Nagai

  • 1Department of Restorative Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan.

American Journal of Dentistry
|August 5, 2009
PubMed
Summary

Two dentin desensitizing agents effectively blocked liquid flow through human dentin discs in vitro. Both MS Coat and Gluma Desensitizer significantly reduced dentin permeability, confirming their efficacy in preventing fluid movement.

Related Experiment Videos

Last Updated: Jun 21, 2026

Inducing Apical Periodontitis in Mice
10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

Area of Science:

  • Dentistry
  • Biomaterials Science
  • Fluid Dynamics

Background:

  • Dentin hypersensitivity is a common clinical condition.
  • Reducing dentin fluid flow is a key mechanism for desensitizing agents.
  • Understanding the in vitro efficacy of desensitizing agents is crucial for product development.

Purpose of the Study:

  • To evaluate the in vitro effectiveness of two dentin desensitizing products in reducing liquid permeability through human dentin.
  • To test the hypothesis that different chemical mechanisms of desensitizing agents yield similar reductions in dentin fluid flow.

Main Methods:

  • Human dentin discs were prepared and their permeability measured in a split chamber model using a chemiluminescence technique.
  • Two desensitizing agents, MS Coat (oxalate-based) and Gluma Desensitizer (glutaraldehyde/HEMA-based), were applied.
  • Permeability was assessed under two pressure cycles after EDTA treatment (control) and albumin soaking.

Main Results:

  • Dentin discs treated with MS Coat and Gluma Desensitizer showed a significant reduction in liquid permeability.
  • Permeability was reduced to near zero levels for both agents at both tested pressures (P < 0.001).
  • The efficacy of both agents in blocking fluid flow was comparable.

Conclusions:

  • Both MS Coat and Gluma Desensitizer are highly effective in reducing dentin liquid permeability in vitro.
  • The tested desensitizing agents, despite differing mechanisms, demonstrated similar efficacy in eliminating fluid flow through dentin.
  • These findings support the use of these agents for managing dentin hypersensitivity.