Related Experiment Video
Updated: Jun 21, 2026
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
[CD11b expression in neutrophils and lymphocytes of children with systemic inflammatory response syndrome]
Wei-Dong Huang1, Jing-Tao Guo, Xi Liu
1Department of Pediatrics, Shenzhen Bao'an District Maternal and Child Health Care Hospital, Shenzhen, Guangdong 518133, China. wdhuang126@163.com
Insights
Neutrophil CD11b expression is a reliable indicator for diagnosing systemic inflammatory response syndrome (SIRS) in children. Conversely, decreased lymphocytic CD11b expression may signal worsening SIRS conditions.
Area of Science:
- Pediatric immunology
- Critical care medicine
- Flow cytometry applications
Background:
- Systemic inflammatory response syndrome (SIRS) is a critical condition in children.
- Understanding immune cell markers is crucial for SIRS diagnosis and management.
Purpose of the Study:
- To evaluate the diagnostic significance of CD11b expression in neutrophils and lymphocytes in pediatric SIRS.
- To determine the correlation between CD11b levels and SIRS severity.
Main Methods:
- Flow cytometry was used to measure CD11b expression in neutrophils and lymphocytes.
- Study included 36 children with SIRS and 28 controls with infectious disease but no SIRS.
- Sensitivity and specificity of neutrophil CD11b for SIRS diagnosis were assessed.
Main Results:
- Neutrophil CD11b expression was significantly higher in SIRS patients (96.7%) versus controls (85.1%).
- Neutrophil CD11b >92.2% showed high sensitivity (97.2%) and specificity (92.9%) for SIRS diagnosis.
- Lymphocytic CD11b expression was lower in SIRS patients (13.4%) than controls (19.2%), particularly suppressed in severe sepsis (7.27%).
Conclusions:
- Neutrophil CD11b expression is a valuable biomarker for diagnosing pediatric SIRS.
- Down-regulation of lymphocytic CD11b may indicate disease progression in pediatric SIRS.
Objective:
To investigate the significance of CD11b expression in neutrophils and lymphocytes in children with systemic inflammatory response syndrome (SIRS).
Methods:
CD11b expression in neutrophils and lymphocytes was measured using flow cytometry in 36 children with SIRS (SIRS group) and 28 children with infectious disease but without SIRS (control group). The sensitivity and specificity of neutrophil CD11b for diagnosis of SIRS were evaluated.
Results:
During the acute phase, an increased CD11b expression in neutrophils (96.7+/-8.1%) was observed in the SIRS group compared with the control group (85.1+/-5.1%) (p<0.05). Using neutrophil CD11b expression >92.2% as a cut-off value for diagnosis of SIRS, the sensitivity and the specificity were 97.2 % and 92.9% respectively. Lymphocytic CD11b expression in the SIRS group (13.4+/-8.6%) was lower than that in the control group (19.2+/-6.4%) in the acute phase (p<0.05). In the SIRS group, lymphocytic CD11b expression was remarkably suppressed in the severe sepsis subgroup (7.27+/-3.04%), showing significantly decreased expression compared with the non-infectious subgroup (19.3+/-2.9%) and the sepsis subgroup (15.9+/-12.5%) (p<0.01). In the convalescence stage lymphocytic CD11b expression in the SIRS group was similar to that in the control group.
Conclusions:
CD11b expression in neutrophils may serve as a reliable indicator for diagnosis of SIRS. The down-regulation of lymphocytic CD11b expression might be a signal of the condition aggravation in children with SIRS.
