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Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
Published on: April 4, 2019
Mammalian reoviruses: propagation, quantification, and storage
Alicia Berard1, Kevin M Coombs
1University of Manitoba and Manitoba Centre for Proteomics and Systems Biology, Winnipeg, Manitoba, Canada.
Current Protocols in Microbiology
|August 5, 2009
Summary
This study details methods for culturing and handling mammalian reoviruses, common pathogens causing mild infections. These techniques are crucial for studying reoviruses and related viruses in the Reoviridae family.
Area of Science:
- Virology
- Cell biology
Background:
- Mammalian reoviruses are ubiquitous pathogens causing gastrointestinal and respiratory infections.
- Reoviruses represent the Reoviridae family, which includes highly pathogenic viruses.
- Most humans develop immunity to mammalian reoviruses early in life.
Purpose of the Study:
- To describe essential techniques for mammalian reovirus research.
- To provide protocols for cell line culturing, virus propagation, and quantification.
Main Methods:
- Culturing of mouse fibroblast L929 cell lines.
- Mammalian reovirus propagation and quantification.
- Virus purification and storage protocols.
Main Results:
- Established protocols for working with mammalian reoviruses in L929 cells.
- Detailed methods for reovirus propagation, quantification, purification, and storage.
Conclusions:
- The described techniques are fundamental for mammalian reovirus research.
- Standardized methods facilitate consistent and reproducible studies on reoviruses.
