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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 21, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Tag-extension-based method for sensitive and specific genotyping of single nucleotide polymorphism on microarray.

Qun Shan¹, Yuanlin Zheng, Guoqing Chen

¹Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Xuzhou Normal University, Xuzhou 221116, China.

Clinica Chimica Acta; International Journal of Clinical Chemistry

|August 6, 2009

Summary

This study introduces a novel microarray method for high-throughput SNP genotyping. The tag extension strategy offers enhanced sensitivity and specificity for genetic analysis.

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Published on: November 19, 2013

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

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Published on: June 21, 2018

Area of Science:

Genetics
Molecular Biology
Bioinformatics

Background:

The increasing volume of single nucleotide polymorphism (SNP) data necessitates advanced genotyping methods.
Current SNP genotyping strategies require improvements in sensitivity, accuracy, and throughput.

Purpose of the Study:

To develop and validate a reliable microarray-based method for high-throughput SNP genotyping.
To address the demand for sensitive and accurate SNP identification.

Main Methods:

A tag extension strategy utilizing fluorescent nucleotide incorporation.
Hybridization of allele-specific primers to a bi-functional linker on a 3D polyacrylamide gel microarray.
Simultaneous incorporation of multiple fluorescence-labeled deoxynucleotides (dNTPs) into the tagged linker.

Main Results:

The developed method demonstrated significantly enhanced sensitivity and specificity in SNP genotyping.
Accurate discrimination of 8025575C/G polymorphisms in the GABAB3 gene across 89 samples.
Validation of results using Sanger sequencing confirmed the method's accuracy.

Conclusions:

The tag-extension-based microarray method is a viable high-throughput tool for obtaining SNP information.
This approach offers a reliable and efficient solution for genetic analysis and SNP discovery.