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Spheroid Assay to Measure TGF-β-induced Invasion
Published on: November 16, 2011
CD26 expression on T cell lines increases SDF-1-alpha-mediated invasion.
1Division of Hematology/Oncology, University of Florida, Gainesville, FL 32610, USA.
British Journal of Cancer
|August 6, 2009
Summary
CD26 expression in T cells enhances invasion mediated by SDF-1-alpha, a process involving PI-3K and MEK1 pathways. CD45 may also play a role in CD26-driven cell invasion.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- CD26 is a glycoprotein involved in tumor growth.
- CD26 expression correlates with aggressiveness in T-cell malignancies.
Purpose of the Study:
- Investigate the role of CD26 in T-cell invasiveness.
- Determine the molecular pathways regulating CD26-mediated invasion.
Main Methods:
- In vitro invasion assays using T-cell lines with varying CD26 expression.
- Flow cytometry and western immunoblotting for protein analysis.
- Zymography to assess enzyme activity and inhibitor effects.
Main Results:
- CD26 expression enhanced SDF-1-alpha-mediated T-cell invasion.
- PI-3K and MEK1 pathways regulate invasion via Akt and p44/42 MAP kinase phosphorylation.
- CD45 inhibition reduced CD26-associated invasion.
Conclusions:
- CD26 upregulates SDF-1-alpha-induced invasion in T cells through PI-3K and MEK1 pathways.
- CD45 may mediate CD26's role in invasion, requiring further investigation.
