Related Experiment Video
Updated: Jun 21, 2026

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis
Published on: September 27, 2019
A low-cost, linear, DC - 35 MHz, high-power LED driver for continuous wave (CW) and fluorescence lifetime imaging
Sylvain Gioux1, Vida Kianzad, Razvan Ciocan
1Boston University, Boston, MA.
Abstract:
Near-infrared (NIR) fluorescence has the potential to provide surgeons with real-time intraoperative image-guidance. Increasing the signal-to-background ratio of fluorescent agents involves delivering a controllable excitation fluence rate of proper wavelength and/or using complementary imaging techniques such as FLIM. In this study we describe a low-cost linear driver circuit capable of driving Light Emitting Diodes (LEDs) from DC to 35 MHz, at high power, and which permit fluorescence CW and lifetime measurements. The electronic circuit Gerber files described in this article and the list of components are available online at www.frangionilab.org.
Related Concept Videos
Fluorescence and Phosphorescence: Instrumentation
Confocal Fluorescence Microscopy

