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A dot immunobinding assay on nitrocellulose for the detection of bovid herpesvirus-4 antibodies

K Naeem1, S M Goyal

  • 1Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, Saint Paul 55108.

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
|April 1, 1990
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.