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Updated: Feb 8, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
Published on: November 22, 2024
Influence of recombinant human platelet-derived growth factor on lateral ridge augmentation using biphasic calcium
Frank Schwarz1, Martin Sager, Daniel Ferrari
1Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany. frank.schwarz@med.uni-duesseldorf.de
Background:
The present study investigated the effects of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on initial bone formation following lateral ridge augmentation using biphasic calcium phosphate (BCP) in combination with a native collagen membrane (CM) in dogs.
Methods:
Eight chronic-type mandibular lateral ridge defects in four beagle dogs (two defects each) were randomly allocated in a split-mouth design to BCP + rhPDGF-BB + CM (test) or BCP + CM (control). After 3 weeks, dissected blocks were prepared for immunohistochemical (angiogenesis-transglutaminase II [TG]) and histomorphometric analysis (e.g., augmented area [AA] and mineralized tissue [MT]). The biologic activity of rhPDGF-BB soak-loaded BCP was verified by means of alkaline phosphatase and lactate dehydrogenase assays in a SaOs-2-osteoblast-like cell culture.
Results:
Both groups exhibited a comparable pattern of blood vessel and bone formation that mainly originated from the adjacent alveolar bone. However, test sites revealed a pronounced TG antigen reactivity, which was associated with statistically significant higher mean AA (8.5 +/- 0.9 mm(2) versus 7.1 +/- 1.1 mm(2)) and MT (2.7 +/- 0.9 mm(2) versus 1.7 +/- 0.8 mm(2)) values compared to the control sites (P <0.05; paired t test).
Conclusion:
BCP + rhPDGF-BB might have the potential to support the initial stages of guided bone regeneration at chronic-type lateral ridge defects.
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