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Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Published on: September 20, 2021

From GH to Billy Ghrelin.

¹Department of Metabolism and Aging, The Scripps Research Institute Florida, Jupiter, FL 33458, USA. rgsmith@scripps.edu

Cell Metabolism

|August 7, 2009

Summary

This summary is machine-generated.

Ghrelin O-acyltransferase (GOAT) activity, crucial for ghrelin octanoylation, changes with fasting and medium-chain triglycerides (MCT). This impacts growth hormone release and energy balance regulation.

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Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

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Area of Science:

Endocrinology
Metabolism
Molecular Biology

Background:

Ghrelin is an octanoylated peptide hormone regulating growth hormone release and energy balance.
Ghrelin O-acyltransferase (GOAT) is essential for ghrelin octanoylation.

Purpose of the Study:

To investigate the in vivo regulation of ghrelin O-acyltransferase (GOAT) activity.
To determine the effects of fasting and medium-chain triglycerides (MCT) on GOAT activity.

Main Methods:

Utilized genetically modified mice models.
Monitored GOAT activity under different nutritional conditions (fasting and MCT ingestion).

Main Results:

In vivo GOAT activity was found to decrease during fasting periods.
Ingestion of medium-chain fatty acid triglycerides (MCT) led to an increase in GOAT activity.

Conclusions:

Nutritional status significantly influences ghrelin octanoylation via GOAT activity.
Dietary medium-chain fatty acid triglycerides (MCT) can modulate ghrelin signaling pathways.