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Alteration of cell surfaces with increased tumorigenicity
Archivum Immunologiae Et Therapiae Experimentalis
|January 1, 1977
Summary
Repeatedly passing transformed mouse macrophages through mice increases their tumor-forming ability. This change correlates with altered cell growth, increased Concanavalin A (Con A) agglutination, and surface morphology modifications observed via scanning electron microscopy (SEM).
Area of Science:
- Cell Biology
- Oncology
- Immunology
Background:
- Tumorigenic cell lines are crucial models for cancer research.
- Understanding the factors influencing tumor progression in vivo is essential.
Purpose of the Study:
- To compare tumorigenicity of mouse macrophage cell lines.
- To investigate the association between cell passage, growth characteristics, agglutinability, and surface morphology.
Main Methods:
- Utilized four mouse tumorigenic cell lines derived from transformed peritoneal macrophages.
- Assessed tumorigenicity, saturation densities, and Concanavalin A (Con A) agglutinability.
- Examined surface morphology using scanning electron microscopy (SEM) after repeated passages in syngeneic mice.
Main Results:
- Increased tumorigenicity after repeated passage was observed.
- Associated changes included altered growth characteristics and higher Con A agglutinability.
- SEM revealed progressive changes in cell surface morphology correlating with increased tumorigenicity.
Conclusions:
- Repeated in vivo passage enhances the tumorigenic potential of transformed macrophages.
- Cellular changes in growth, agglutination, and surface structure accompany enhanced tumorigenicity.