Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Further studies on human enteric coronaviruses.

E O Caul, S I Egglestone

    Archives of Virology
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Human enteric coronaviruses were compared to animal strains using negative staining. Researchers observed virus replication in human intestinal cells, identifying particles and a potential viral nucleocapsid structure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Epidemiological, social, diagnostic and economic evaluation of population screening for genital chlamydial infection.

    Health technology assessment (Winchester, England)·2007
    Same author

    Borna disease virus and mental health: a cross-sectional study.

    QJM : monthly journal of the Association of Physicians·2005
    Same author

    National epidemic of Lordsdale Norovirus in the UK.

    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2004
    Same author

    A school outbreak of Norwalk-like virus: evidence for airborne transmission.

    Epidemiology and infection·2003
    Same author

    Measurement of IgG antibodies to Chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed Chlamydia psittaci/Chlamydia pneumoniae infection.

    Journal of clinical pathology·2003
    Same author

    A novel polymerase chain reaction assay to detect Mycoplasma genitalium.

    Molecular pathology : MP·2003

    Area of Science:

    • Virology
    • Gastroenterology
    • Microbiology

    Background:

    • Enteric coronaviruses infect humans, pigs, and calves.
    • Understanding their replication is crucial for public health.

    Purpose of the Study:

    • To compare human enteric coronaviruses with those from pigs and calves.
    • To investigate the replication of human enteric coronavirus in intestinal organ cultures.

    Main Methods:

    • Negative staining electron microscopy was used for comparisons.
    • Human intestinal organ cultures were inoculated and examined over time.
    • Thin section electron microscopy was employed to study cellular changes and virus morphology.

    Main Results:

    • Increasing numbers of viral particles were observed in culture fluids post-inoculation.

    Related Experiment Videos

  • Columnar epithelial cells showed features consistent with coronavirus replication.
  • Virus particles measured approximately 68 nm in diameter.
  • A novel structure, possibly the viral nucleocapsid, was identified in thin sections.

    • Conclusions:

    • Human enteric coronaviruses share similarities with animal enteric coronaviruses.
    • Evidence suggests human enteric coronavirus replicates within human intestinal epithelial cells.
    • A previously undescribed structure may be the viral nucleocapsid, warranting further investigation.