Related Experiment Video
Updated: Jun 21, 2026
06:28
Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
Published on: June 4, 2020
Multicentric comparison of single portion reagents and liquid reagents for thromboelastometry
Niels Rahe-Meyer1, Cristina Solomon, Matthias Vorweg
1Department of Anesthesia, Hannover Medical School, 30625 Hannover, Germany.
Summary
New single portion reagents (SPR) for thromboelastometry ROTEM offer a simpler, more stable alternative to traditional liquid reagents. This innovation streamlines whole blood clotting assessments in clinical settings.
Area of Science:
- Hemostasis and Thrombosis
- Clinical Diagnostics
- Biochemistry
Background:
- Thromboelastometry ROTEM is a viscoelastic whole blood clotting assessment tool.
- Current liquid reagents require multiple pipetting steps, complicating clinical use.
- New single portion reagents (SPR) consolidate lyophilized reagents into one vial for simplified blood addition.
Purpose of the Study:
- To compare the performance and stability of new single portion reagents (SPR) against standard liquid reagents for ROTEM analysis.
- To evaluate the ease of use and correlation of SPR with conventional methods.
Main Methods:
- Comparison of SPR and liquid reagents across multiple ROTEM tests (EXTEM, INTEM, FIBTEM, APTEM, HEPTEM).
- Analysis of 21 normal and 46 patient blood samples across three study centers.
- Assessment of SPR stability at 37°C using 23 blood samples.
Main Results:
- Excellent correlation (0.85-0.99) found between SPR and liquid reagents for all clotting parameters.
- SPR demonstrated excellent stability under thermal stress.
- Measurements using SPR were significantly easier to perform than with liquid reagents.
Conclusions:
- Single portion reagents (SPR) provide a reliable and user-friendly alternative to liquid reagents for ROTEM analysis.
- SPR simplify the process of whole blood clotting assessment, enhancing clinical routine efficiency.
- The stability and ease of use of SPR support their adoption in hemostasis and hematology diagnostics.
More Related Videos
Related Concept Videos
01:23EDTA: Indirect and Alkalimetric Titration
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
01:30EDTA: Direct, Back-, and Displacement Titration
The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides and...
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides and...