Related Experiment Video
Updated: Jun 21, 2026
Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia
Published on: September 15, 2023
Three-color intranuclear staining for measuring mitosis and apoptosis in cells transfected with a GFP-tagged histone
J Rios-Doria1, J Meléndez, A N Monteiro
1Risk Assessment, Detection, and Intervention Program, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL 33612, USA.
Abstract:
Green fluorescent protein (GFP) has become a powerful tool for monitoring the expression of transfected genes by flow cytometry including GFP-tagged histones for tracking chromatin and elucidating histone function. We describe here a method for simultaneous detection of three nucleus-localized signals: a GFP-tagged histone, DNA content and detection of phosphorylated histone H3, which labels mitotic cells. We also demonstrate another application of this method for simultaneous detection of a GFP-tagged histone, DNA content, and cleaved caspase-3.
