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Updated: Jun 21, 2026
Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
Published on: September 16, 2021
Human oocyte cryopreservation: evidence for practice
1Centre for Reproductive Medicine and Fertility, The Jessop Wing, Sheffield Teaching Hospitals NHS Trust, Sheffield, S10 2SF, UK. rachel.cutting@sth.nhs.uk
Aim:
The aim of this article is to review the evidence regarding human oocyte cryopreservation by slow freezing and vitrification and to provide evidence-based clinical and laboratory guidelines on the effectiveness and safety of these technologies.
Scope:
These guidelines will address the following: Stage of oocyte maturity. Methods of cryopreservation/warming. Slow controlled cooling. Vitrification. Techniques used for insemination. Ovarian stimulation regimens. The guidelines do not cover recommendations for ovarian tissue freezing or in vitro oocyte maturation (IVM).
Outcome Measures:
The following outcome measures were examined: cryosurvival, fertilisation rate, cleavage rate, clinical pregnancy rate and implantation rate, health of resulting children.
