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Published on: December 22, 2017
Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor
Anita Zamboni1, Pamela Gatto, Alessandro Cestaro
1IASMA Research and Innovation Center, Fondazione Edmund Mach, S. Michele a/Adige I-38010, Italy. anita.zamboni@univr.it
Background:
In response to pathogen attack, grapevine synthesizes phytoalexins belonging to the family of stilbenes. Grapevine cell cultures represent a good model system for studying the basic mechanisms of plant response to biotic and abiotic elicitors. Among these, modified beta-cyclodextrins seem to act as true elicitors inducing strong production of the stilbene resveratrol.
Results:
The transcriptome changes of Vitis riparia x Vitis berlandieri grapevine cells in response to the modified beta-cyclodextrin, DIMEB, were analyzed 2 and 6 h after treatment using a suppression subtractive hybridization experiment and a microarray analysis respectively. At both time points, we identified a specific set of induced genes belonging to the general phenylpropanoid metabolism, including stilbenes and hydroxycinnamates, and to defence proteins such as PR proteins and chitinases. At 6 h we also observed a down-regulation of the genes involved in cell division and cell-wall loosening.
Conclusions:
We report the first large-scale study of the molecular effects of DIMEB, a resveratrol inducer, on grapevine cell cultures. This molecule seems to mimic a defence elicitor which enhances the physical barriers of the cell, stops cell division and induces phytoalexin synthesis.
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