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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

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Related Experiment Video

Updated: Jun 21, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Dissecting protein function and signaling using protein microarrays.

Alejandro Wolf-Yadlin1, Mark Sevecka, Gavin MacBeath

  • 1Department of Chemistry & Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.

Current Opinion in Chemical Biology
|August 8, 2009
PubMed
Summary

Protein microarrays enable high-throughput study of protein interactions and modifications. Further development is needed for rigorous quantitative data and reagent selectivity to advance cell biology understanding.

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Last Updated: Jun 21, 2026

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Studying protein recognition and signaling in isolation is common, but challenging at system-wide or proteome-wide scales within biological complexity.
  • Protein microarray technology offers a powerful approach for high-throughput analysis of protein-protein interactions and quantification of protein states.

Purpose of the Study:

  • To provide an overview of protein microarray applications.
  • To compare the strengths and technical challenges of different protein microarray approaches.
  • To highlight areas for improvement in protein microarray technology.

Main Methods:

  • Review and comparison of various protein microarray applications.
  • Analysis of strengths and technical challenges inherent in protein microarray techniques.
  • Discussion of reagent selectivity and quantitative data acquisition.

Main Results:

  • Protein microarrays are versatile tools for high-throughput assessment of protein-protein interactions.
  • They allow quantification of protein abundances and post-translational modifications in complex mixtures.
  • Current challenges include obtaining rigorously controlled quantitative data and assessing reagent selectivity.

Conclusions:

  • Protein microarray technology has significant potential to impact cell biology and physiology.
  • Increased emphasis on rigorously controlled quantitative data from protein function microarrays is crucial.
  • Assessing the selectivity of reagents used with protein-detecting microarrays is essential for reliable results.