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Brain Infarct Segmentation and Registration on MRI or CT for Lesion-symptom Mapping
Published on: September 25, 2019
Intense correlation between brain infarction and protein-conjugated acrolein
Ryotaro Saiki1, Kazuhiro Nishimura, Itsuko Ishii
1Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan.
Stroke
|August 8, 2009
Summary
Protein-conjugated acrolein increases at the stroke site and in plasma, while spermine and spermidine decrease. This finding correlates with stroke induction and suggests acrolein
Area of Science:
- Biochemistry
- Neuroscience
- Pathology
Background:
- Elevated plasma protein-conjugated acrolein and polyamine oxidases indicate stroke.
- Acrolein is produced by polyamine oxidases from substrates like spermine and spermidine.
Purpose of the Study:
- To investigate if protein-conjugated acrolein increases and spermine/spermidine decrease at the infarction site.
- To confirm the role of acrolein in stroke pathology.
Main Methods:
- Mouse stroke model induced by photoinduction with Rose Bengal.
- Analysis of infarction volume using NIH image analysis software.
- Measurement of protein-conjugated acrolein and polyamines via Western blotting, ELISA, and HPLC.
Main Results:
- Protein-conjugated acrolein significantly increased at the infarction site 24 hours post-stroke.
- Spermine and spermidine levels decreased at the infarction site.
- N-acetylcysteine, an acrolein scavenger, reduced infarction size.
- Increased plasma levels of protein-conjugated acrolein, polyamines, and polyamine oxidases were observed post-stroke.
Conclusions:
- Stroke induction correlates with increased protein-conjugated acrolein at the infarction site and in plasma.
- Acrolein plays a significant role in stroke pathology.
