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Structure and Function of Platelets

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Related Experiment Video

Updated: Jun 21, 2026

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

Foxp3 regulates megakaryopoiesis and platelet function.

Jamie J Bernard1, Kathryn E Seweryniak, Anne D Koniski

  • 1Department of Environmental Medicine, University of Rochester, Rochester, NY 14642, USA.

Arteriosclerosis, Thrombosis, and Vascular Biology
|August 8, 2009
PubMed
Summary

The transcription factor Foxp3 is essential for megakaryocyte development and platelet function. Its absence causes platelet abnormalities and proliferation defects in megakaryocyte progenitors, impacting hemostasis and inflammation.

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Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
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Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

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Last Updated: Jun 21, 2026

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09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow
07:58

In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow

Published on: July 28, 2021

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
14:30

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Area of Science:

  • Hematology
  • Immunology
  • Molecular Biology

Background:

  • Platelets are critical for hemostasis and inflammation regulation.
  • Foxp3 is a key transcription factor for T regulatory cell development.
  • Mutations in Foxp3 cause IPEX syndrome and scurfy mouse models, leading to autoimmunity, skin diseases, and thrombocytopenia.

Purpose of the Study:

  • To investigate the role of functional Foxp3 in megakaryocyte development and platelet biology.
  • To determine if Foxp3 deficiency leads to defects in megakaryocytes and platelets.

Main Methods:

  • Detection of Foxp3 mRNA and protein in human and mouse megakaryocytes.
  • Utilized shRNA and Foxp3-deficient (Foxp3(sf)) mice to study Foxp3's function.
  • Analyzed platelet function, including spreading and release of specific factors, in IPEX patients and Foxp3(sf) mice.

Main Results:

  • Foxp3 is expressed in human and mouse megakaryocytes.
  • Foxp3-deficient megakaryocyte progenitors show proliferation defects.
  • Observed significant platelet abnormalities in an IPEX patient and Foxp3(sf) mice, including impaired spreading and altered release of TGF-beta and CD40 ligand (CD40L).
  • Foxp3(sf) mice exhibited thrombocytopenia, increased platelet volume, and altered serum levels of CD40L, TXB(2), and TGF-beta.

Conclusions:

  • Foxp3 is essential for proper megakaryopoiesis (megakaryocyte development).
  • Foxp3 plays a crucial role in regulating platelet function, including spreading and mediator release.
  • These findings highlight Foxp3 as a significant factor in platelet biology and hemostasis.