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Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Overview of Protein Sorting and Transport01:45

Overview of Protein Sorting and Transport

Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation.  In gated transport, folded...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
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Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

Predicting subcellular location of proteins using integrated-algorithm method.

Yu-Dong Cai1, Lin Lu, Lei Chen

  • 1Institute of System Biology, Shanghai University, 99 ShangDa Road, 200244 Shanghai, China. cyd@picb.ac.cn

Molecular Diversity
|August 8, 2009
PubMed
Summary
This summary is machine-generated.

Predicting protein subcellular location is crucial for various biological applications. This study developed an integrated algorithm achieving 80.56% accuracy on a test set, outperforming existing methods.

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Last Updated: Jun 21, 2026

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
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Published on: August 8, 2025

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An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Area of Science:

  • Biochemistry
  • Bioinformatics
  • Computational Biology

Background:

  • Protein subcellular localization is vital for understanding protein function, genome annotation, and drug design.
  • Experimental determination of protein location is time-consuming and not high-throughput.
  • Accurate prediction of protein subcellular location is essential for large-scale biological studies.

Purpose of the Study:

  • To develop and evaluate integrated-algorithm methods for high-throughput protein subcellular location prediction.
  • To improve the accuracy and efficiency of predicting where proteins reside within a cell.
  • To provide a reliable computational tool for protein localization prediction.

Main Methods:

  • Utilized two datasets (2,391 training, 2,598 testing proteins) from Chou and Elrod (1999).
  • Represented protein sequences using amino acid composition.
  • Developed an integrated predictor by combining 10 Weka algorithms using the Minimum Redundancy Maximum Relevance (mRMR) method.
  • Employed jackknife cross-validation for performance testing.

Main Results:

  • The best integrated-algorithm predictor achieved a correct rate of 77.83% on the training set and 80.56% on the test set.
  • The developed predictor outperformed all 60 individual algorithms available in Weka.
  • The method demonstrates significant improvement over existing computational approaches.

Conclusions:

  • The developed integrated-algorithm predictor offers a highly accurate and efficient method for determining protein subcellular location.
  • This computational tool can significantly aid in protein function prediction, genome annotation, and drug design.
  • The predicting software is available upon request, facilitating further research.