Related Experiment Video
Updated: Jun 21, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
Published on: June 13, 2010
A rapid isolation and identification method for blocked N-terminal peptides by isothiocyanate-coupled magnetic
Liyan Zhao1, Yangjun Zhang, Junying Wei
1State Key Lab of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, P. R. China.
Abstract:
A quick isolation and identification of N-blocked peptides from protein digest mixtures were achieved by diisothiocyanate or isothiocyanate-coupled magnetic nanoparticles and MS. After protein digests were guanidinated and then mixed with diisothiocyanate or isothiocyanate-coupled magnetic nanoparticles, unmodified N-terminal peptides were covalently bound to magnetic nanoparticles, and can be removed from the mixture under magnetic field. Therefore, N-blocked peptides could be isolated and analyzed by MALDI or ESI MS. This new strategy was demonstrated with model peptides, proteins, and the lysates of HepG2 cells.
Related Concept Videos
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Rapid Identification of Pathogens

