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The effect of oral and subcutaneous meperidine on the maximal electroshock seizure (MES) in mice
D O Yillar1, A G Akkan, A Akcasu
1University of Istanbul, Cerrahpaşa Medical Faculty, Department of Pharmacology and Clinical Pharmacology 34303 Cerrahpaşa, Istanbul, Turkey. oyillar@istanbul.edu.tr
Abstract:
The likely effect of oral and subcutaneous meperidine on maximal electroshock seizure (MES) in mice was studied. Convulsive current fifty (CC50) was assessed to be 46m A, an electrical pulse causing seizure in 50% of test animals. Doses of 15, 30, 60, or 120 mg/kg meperidine given orally or subcutaneously increased the convulsion threshold of MES as evidenced by a significant dose-dependent reduction of MES below control value (p < .05). An initial hyperactivity reaction that was worsened by noisy and tactile stimuli and tail erection followed by sedation was observed after s.c. injection of 60 or 120 mg/kg meperidine. No significant difference was found between meperidine-induced reductions of control MES values obtained one and two hours after oral doses; the depressed MES values obtained one hour after oral administration of meperidine were significantly different and more powerful than those obtained two hours after s.c. drug administrations (p < .05). Combining previous literature information with the present results, we conclude that such an effect of meperidine can be attributed to cerebellar stimulation.
Insights
Meperidine, administered orally or subcutaneously, raises the seizure threshold in mice. This effect, likely due to cerebellar stimulation, was dose-dependent and more pronounced with oral administration.
Area of Science:
- Neuroscience
- Pharmacology
Background:
- Maximal electroshock seizure (MES) is a standard model for evaluating anticonvulsant properties.
- Meperidine is an opioid analgesic with potential central nervous system effects.
Purpose of the Study:
- To investigate the anticonvulsant effects of oral and subcutaneous meperidine on MES in mice.
- To determine the dose-response relationship and time course of meperidine's effect on seizure threshold.
Main Methods:
- Mice were administered varying doses of meperidine orally or subcutaneously.
- The convulsive current fifty (CC50) was determined to assess the seizure threshold.
- Behavioral observations, including hyperactivity and sedation, were recorded.
Main Results:
- Meperidine significantly increased the MES convulsion threshold in a dose-dependent manner.
- Oral meperidine showed a more potent and sustained reduction in MES values compared to subcutaneous administration.
- Observed behavioral effects included initial hyperactivity followed by sedation.
Conclusions:
- Meperidine exhibits anticonvulsant properties, increasing the seizure threshold in mice.
- The findings suggest that cerebellar stimulation may underlie meperidine's effect on MES.
- Route of administration influences the efficacy and duration of meperidine's anticonvulsant action.
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