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Synthesis of an In vivo MRI-detectable Apoptosis Probe
Published on: July 31, 2012
Translational imaging of apoptosis
Graham Smith1, Quang-Dé Nguyen, Eric O Aboagye
1Comprehensive Cancer Imaging Centre, Faculty of Medicine, Imperial College London, Hammersmith Hospital, Du Cane Road, London, W12 0NN, UK.
Abstract:
Deregulated apoptosis is involved in several diseases including myocardial infarction, ischemia and neurodegenerative disorders, which are characterized by excessive apoptosis. In contrast, resistance to apoptosis is defined as one of the hallmarks of cancer. It therefore follows that strategies that enable the quantitative detection of apoptosis modulation in vivo would be of enormous benefit in the clinic for diagnosis and patient management (evaluation of response to treatment). In addition, such strategies could be used to evaluate the efficacy of novel therapeutics along their development process. During the development of novel therapeutics it would be necessary to evaluate drug efficacy in vitro and then in experimental animal models and, ultimately, in clinical trials. Currently there is no one single probe that is suitable for imaging apoptosis at every stage of evaluation, necessitating a switch between probe types during the development process. This has key implications for the quality and reproducibility of the data obtained. The present review summarizes the development of new apoptosis detecting probes that have the potential for bridging different stages of the evaluation process such that accurate, translational apoptosis imaging data are obtained.
Insights
Quantitative detection of apoptosis modulation is crucial for diagnosing diseases and evaluating cancer treatments. New probes aim to provide consistent imaging across all research and clinical stages, improving data quality and reproducibility.
Area of Science:
- Biomedical imaging
- Molecular biology
- Disease diagnostics
Background:
- Deregulated apoptosis (programmed cell death) is implicated in diseases like myocardial infarction, ischemia, neurodegenerative disorders, and cancer.
- Quantitative in vivo apoptosis detection is vital for clinical diagnosis, patient management, and therapeutic development.
Purpose of the Study:
- To review the development of novel apoptosis-detecting probes.
- To identify probes capable of bridging different evaluation stages (in vitro, animal models, clinical trials).
- To enhance the accuracy and reproducibility of translational apoptosis imaging data.
Main Methods:
- Review of current literature on apoptosis imaging probes.
- Analysis of probe suitability for various stages of therapeutic evaluation.
- Focus on probes enabling consistent, quantitative apoptosis detection.
Main Results:
- Current apoptosis imaging necessitates switching probe types during development, impacting data quality.
- New probes are being developed with the potential for cross-stage applicability.
- These novel probes aim to provide accurate, translational apoptosis imaging.
Conclusions:
- Developing a single, versatile probe for apoptosis imaging across all research and clinical stages is a key goal.
- Such probes would improve the quality and reproducibility of data in disease diagnosis and drug development.
- Advancements in apoptosis imaging probes promise more reliable patient management and therapeutic evaluation.
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