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Updated: Jun 21, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Mean centering of ratio kinetic profiles for the simultaneous kinetic determination of binary mixtures in
1School of Chemistry, Damghan University of Basic Sciences, Damghan, Iran. zarei@dubs.ac.ir
Abstract:
In this work, the applicability of mean centering (MC) of ratio kinetic profiles method to the kinetic voltammetry data is verified. For this purpose, a procedure is described for the determination of Sb(III) and Sb(V) by adsorptive linear sweep voltammetry using pyrogallol (py) as a complexing agent. The method is based on the differences between the rate of complexation of pyrogallol with Sb(V) and Sb(III) at pH 1.2. The results show that the mean centering of ratio kinetic profiles method is suitable for the speciation of antimony. Sb(III) and Sb(V) can be determined in the ranges of 3.0-120.0 and 10.0-240.0 ng mL(-1), respectively. Moreover, the solution is analyzed for any possible effects of foreign ions. The obtained results show that the method of MC in combination to electroanalytical techniques is a powerful method with high sensitivity and selectivity. The procedure is successfully applied to the speciation of antimony in pharmaceutical preparations.
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