Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Viscosity01:27

Viscosity

Viscosity is a property of fluids that measures their resistance to flow. It is influenced by factors such as the surface area of contact, the gradient of flow speed, and the fluid's viscosity constant, called the coefficient of viscosity. The coefficient of viscosity, also known as dynamic viscosity, is denoted by the symbol η. It determines the proportionality between the viscous force and the gradient of flow speed.Newton's law of viscosity states that the viscous force on a faster-moving...
In Vitro Drug Dissolution: Alternative Methods01:17

In Vitro Drug Dissolution: Alternative Methods

Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
Surface Tension, Capillary Action, and Viscosity02:57

Surface Tension, Capillary Action, and Viscosity

Surface Tension
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
Theories of Dissolution: Diffusion Layer Model01:15

Theories of Dissolution: Diffusion Layer Model

Dissolution, the process by which drug particles dissolve in a solvent, is explained by the diffusion layer model, a theoretical framework that simulates the absorption of oral drugs and allows us to analyze experimental data.
This process starts with a thin layer, saturated with the drug, forming at the interface between the solid and liquid. The solute then diffuses from this layer into the main solution. The Noyes-Whitney equation suggests that the rate of dissolution relies on the diffusion...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-Assembly of Amyloid Fibrils into Fibrillar Superstructure Monitored with Thioflavin T.

Biomolecules·2026
Same author

A Metal-Free, Dual-Mode Colorimetric and Fluorometric Sensing of Rhodamine-B Using Multifaceted Biogenic Carbon Quantum Dots: A Biocompatible Probe for Biological and Plant Confocal Imaging.

Luminescence : the journal of biological and chemical luminescence·2026
Same author

Biophysical translational posterity of green carbon quantum dots: the unparalleled versatility.

Nanomedicine (London, England)·2024
Same author

Crystal Violet Selectively Detects Aβ Oligomers but Not Fibrils In Vitro and in Alzheimer's Disease Brain Tissue.

Biomolecules·2024
Same author

Helical sulfonyl-γ-AApeptides modulating Aβ oligomerization and cytotoxicity by recognizing Aβ helix.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Isoelectric point (pI)-based phase separation (pI-BPS) purification of elastin-like polypeptides (ELPs) containing charged, biologically active fusion proteins (ELP-FPs).

Biotechnology progress·2023
Same journal

Synthesis of covalent organic frameworks and plasmon-assisted exfoliation for enhanced solar hydrogen production.

Journal of colloid and interface science·2026
Same journal

Efficient hydrogen production and anti-coking via reforming of waste plastics by oxygen vacancy promoted plasma-catalysis.

Journal of colloid and interface science·2026
Same journal

Lanthanum-modulated hollow CuO nanofibers enable selective CO<sub>2</sub> electroreduction to multicarbon products at high current densities.

Journal of colloid and interface science·2026
Same journal

Tris(vinyl dimethylsilyl) phosphate: Enhancing interface stability in high-voltage Li-ion batteries at elevated temperatures.

Journal of colloid and interface science·2026
Same journal

Electron-donor modulated built-in electric fields in Ni<sub>2</sub>P/MoS<sub>2</sub> Heterostructures for accelerated sodium storage kinetics.

Journal of colloid and interface science·2026
Same journal

Porous flexible structure mediated synergistic boost of built-in electric field and photothermal effect for enhanced photocatalysis.

Journal of colloid and interface science·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2026

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

Lysozyme as diffusion tracer for measuring aqueous solution viscosity.

Avanish S Parmar1, Martin Muschol

  • 1Department of Physics, University of South Florida, Tampa, FL 33620, USA.

Journal of Colloid and Interface Science
|August 12, 2009
PubMed
Summary
This summary is machine-generated.

Lysozyme offers a superior method for measuring solution viscosity compared to polystyrene beads, maintaining stability across a wide range of conditions. This protein tracer enables reliable viscosity mapping in biological saline solutions.

More Related Videos

The Diffusion of Passive Tracers in Laminar Shear Flow
08:01

The Diffusion of Passive Tracers in Laminar Shear Flow

Published on: May 1, 2018

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Related Experiment Videos

Last Updated: Jun 21, 2026

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

The Diffusion of Passive Tracers in Laminar Shear Flow
08:01

The Diffusion of Passive Tracers in Laminar Shear Flow

Published on: May 1, 2018

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Area of Science:

  • Biophysics
  • Materials Science

Background:

  • Tracer diffusion is a valuable method for assessing solution viscosity and its response to parameters like pH, temperature, and salt concentration.
  • A key limitation in biological saline solutions is tracer particle aggregation at higher ionic strengths, compromising measurement accuracy.

Purpose of the Study:

  • To compare the efficacy of polystyrene nanobeads and the protein lysozyme as tracer particles for viscosity measurements in saline solutions.
  • To identify a stable tracer particle suitable for broad application in biological fluid viscosity analysis.

Main Methods:

  • Dynamic light scattering was employed to measure tracer diffusion.
  • The colloidal stability and performance of polystyrene beads and lysozyme were evaluated across varying ionic strengths, pH, salt types, and temperatures.

Main Results:

  • Polystyrene beads exhibited flocculation above 100mM ionic strength, limiting their utility.
  • Lysozyme demonstrated excellent colloidal and structural stability, enabling viscosity measurements up to 1M ionic strength across diverse conditions (pH, temperature, salt types).

Conclusions:

  • Lysozyme is a robust and reliable tracer particle for accurate viscosity measurements in biologically relevant saline solutions.
  • The use of lysozyme can be extended to other optical techniques like fluorescence correlation spectroscopy for localized diffusion measurements.