The roles of MCP-1 and protein kinase C delta activation in human eosinophilic leukemia EoL-1 cells

Related Experiment Video

Updated: Jun 21, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Ji-Sook Lee1, Eun Ju Yang, In Sik Kim

  • 1Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon 301-746, Republic of Korea.

Cytokine
|August 12, 2009
PubMed

Insights

Monocyte chemoattractant protein-1 (MCP-1) drives eosinophilic leukemia cell movement and survival. Protein kinase C delta (PKC delta) plays a key role in these MCP-1-mediated effects, impacting eosinophil disorders.

Area of Science:

  • Hematology
  • Immunology
  • Molecular Biology

Background:

  • Idiopathic hypereosinophilic syndrome (HES) involves clonal eosinophil proliferation.
  • The FIP1-like-1-platelet-derived growth factor receptor-alpha (FIP1L1-PDGFRA) fusion gene is crucial in HES pathogenesis.
  • Monocyte chemoattractant protein-1 (MCP-1)/CCL2 and protein kinase C delta (PKC delta) roles in eosinophil biology require further investigation.

Purpose of the Study:

  • To investigate the contribution of MCP-1/CCL2 to chemotactic activity.
  • To explore the role of PKC delta in the human eosinophilic leukemia cell line EoL-1.
  • To elucidate the signaling pathways involved in MCP-1-induced EoL-1 cell migration and survival.

Main Methods:

  • Utilized the human eosinophilic leukemia cell line EoL-1, which expresses CCR2.
  • Stimulated EoL-1 cells with MCP-1 to analyze chemotaxis and intracellular signaling.
  • Investigated the involvement of G(i)/G(o) proteins, phospholipase C (PLC), PKC delta, p38 MAPK, and NF-kappaB.

Main Results:

  • MCP-1 induced significant migration of EoL-1 cells.
  • The MCP-1 signaling pathway involves G(i)/G(o) protein, PLC, PKC delta, p38 MAPK, and NF-kappaB.
  • PKC delta activation inhibited NF-kappaB translocation and suppressed apoptosis in EoL-1 cells by inhibiting caspase 3 and caspase 9.

Conclusions:

  • MCP-1 is a key chemoattractant for eosinophilic leukemia cells.
  • PKC delta acts as an anti-apoptotic molecule in EoL-1 cells and mediates MCP-1-induced cell movement.
  • This study enhances understanding of MCP-1 in eosinophil biology and the pathogenesis of eosinophilic disorders.

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

Related Concept Videos

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...