Related Experiment Video
Updated: Jun 21, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
An explanation of the mineralization mechanism in osteoblasts induced by calcium hydroxide
1Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
Abstract:
Calcium hydroxide (Ca(OH)(2)) has been broadly used in endodontics, including apexification to obtain apical closure by mineralization. However, the detailed mechanism of mineralization induced by Ca(OH)(2) is still unclear. This study focuses on the function of calcium and hydroxyl ions which dissociate from Ca(OH)(2) during the mineralization process. Though primary osteoblasts cultured in the medium without or with 0.025mgml(-1) Ca(OH)(2) did not show mineralization, they did exhibit mineralization when they were cultured with a higher concentration of Ca(OH)(2) (0.25mgml(-1)). Mineralization induced in the presence of 0.25mgml(-1) Ca(OH)(2) was greater at pH 7.4 than at pH 8.5. The high mineralization activity observed under neutral conditions was caused by the prolonged activation of p38 and JNK. Hydroxyl ions did not have any effect on the mineralization. The results demonstrate that calcium ions dissociated from Ca(OH)(2) are critical for inducing the mineralization of osteoblasts.
Related Concept Videos
Osteoclasts in Bone Remodeling
Skeleton and Calcium Homeostasis
The Bone Matrix
Essential Minerals for Bone Health
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Bone Remodeling
Factors Affecting Solubility

