Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Thiazole-Linked <i>N</i>-Hydroxypropanamide Derivatives: Selective HDAC6 Inhibitors with Therapeutic Potential for Neurodegenerative Diseases.

Journal of medicinal chemistry·2026

Same author

AbClick Pro H: Scavenger-Free, Traceless Lys248 Conjugation Via <i>O</i>-Acyl Hydroxamate Chemistry for Homogeneous Antibody-Drug Conjugates (ADCs).

Organic letters·2025

Same author

Chemical evolution of antibody-drug conjugates focused on linker design and conjugation strategies in hematology.

Seminars in hematology·2025

Same author

Identification of a novel compound that induces oligomer disassembly of the pyruvate dehydrogenase E2 complex.

Biochemical and biophysical research communications·2025

Same author

Flavonoid-based dual modulation of GPR120 and PPARγ: A potential therapeutic strategy for metabolic disease intervention.

Bioorganic chemistry·2025

Same author

Site-Selective Anti-PD-L1 Antibody-MMAE Conjugate for Enhanced NSCLC Therapy.

ACS medicinal chemistry letters·2025

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

Cascade enzyme-linked immunosorbent assay (CELISA).

Young-mi Lee¹, Yujin Jeong, Hyo Jin Kang

¹BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon 305-333, Republic of Korea.

Biosensors & Bioelectronics

|August 12, 2009

Summary

A novel cascading enzyme-linked immunosorbent assay (CELISA) enhances signal amplification for disease diagnosis. This improved immunoassay achieves sensitive detection of cancer biomarkers like alpha-fetoprotein and prostate-specific antigen in serum.

More Related Videos

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

Related Experiment Videos

Last Updated: Jun 21, 2026

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

Area of Science:

Biochemistry
Immunology
Biotechnology

Background:

Immunoassays, particularly sandwich ELISA, are vital for disease diagnosis and biochemical detection.
Current ELISA methods often rely on horseradish peroxidase or alkaline phosphatase for signal amplification.
Advanced immunoassays have improved sensitivity by modifying signal amplification strategies.

Purpose of the Study:

To develop a novel signal amplification method for ELISA by incorporating an enzyme-cascading system.
To introduce the cascading enzyme-linked immunosorbent assay (CELISA) for enhanced detection sensitivity.
To evaluate CELISA's effectiveness in detecting specific cancer biomarkers.

Main Methods:

Incorporated an enzyme-cascading system, specifically a trypsinogen-enterokinase combination, into the ELISA.
Developed and applied the cascading enzyme-linked immunosorbent assay (CELISA).
Utilized a colorimetric reagent for signal generation and detection.

Main Results:

CELISA demonstrated successful signal amplification.
Achieved limits-of-detection of 0.1-10pM for alpha-fetoprotein (AFP) and prostate-specific antigen (PSA).
Sensitive detection of AFP and PSA was confirmed in whole human serum samples.

Conclusions:

The study presents the first enzyme cascading step integrated into an ELISA system.
CELISA offers a novel and effective approach for sensitive detection of pathogenic antigens.
This method holds promise for improved disease diagnosis through enhanced immunoassay sensitivity.