Related Experiment Video
Updated: Jun 21, 2026

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
Published on: April 3, 2014
The tinker, tailor, soldier in intracellular B12 trafficking
Ruma Banerjee1, Carmen Gherasim, Dominique Padovani
1Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor, MI 48109-5606, USA. rbanerje@umich.edu
Abstract:
The recognition of eight discrete genetic complementation groups among patients with inherited cobalamin disorders provided early insights into the complexity of a cofactor-processing pathway that supports only two known B(12)-dependent enzymes in mammals. With the identification of all eight genes now completed, biochemical interrogations of their functions have started and are providing novel insights into a trafficking pathway involving porters that tinker with and tailor the active cofactor forms and editors that ensure the fidelity of the cofactor loading process. The principles of sequestration and escorted delivery of a rare and reactive organometallic cofactor that are emerging from studies on B(12) might be of general relevance to other cofactor trafficking pathways.
Related Concept Videos
Protein Transport to the Inner Chloroplast Membrane
Protein Transport into the Inner Mitochondrial Membrane
Transport of mitochondrial precursors across the TIM23 channel is driven by...
Intracellular Movement of Viruses and Bacteria
Export of Misfolded Proteins out of the ER
Sulfur Assimilation
Bacterial Translocation and Protein Secretion

