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Updated: Jun 21, 2026
05:17
Preparation of High-Quality Fermented Fish Product
Published on: August 23, 2019
Agromyces atrinae sp. nov., isolated from fermented seafood
Eun-Jin Park1, Min-Soo Kim2,1, Mi-Ja Jung1
1Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Summary
A novel bacterial species, Agromyces atrinae sp. nov., was identified from fermented seafood. This Gram-positive, aerobic rod exhibits unique biochemical properties and represents a new member of the Agromyces genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Fermented seafood harbors diverse microbial communities.
- Identification of novel bacterial species is crucial for understanding microbial diversity and potential applications.
Purpose of the Study:
- To isolate and characterize a novel bacterial species from fermented seafood.
- To determine the phylogenetic and taxonomic position of the novel isolate.
Main Methods:
- Bacterial isolation and cultivation under various conditions (NaCl, pH, temperature).
- Physicochemical characterization including Gram staining, morphology, and motility.
- Analysis of chemotaxonomic markers: menaquinones, fatty acids, cell-wall sugars, peptidoglycan amino acids, and polar lipids.
- Genomic DNA G+C content determination.
- Phylogenetic analysis based on 16S rRNA gene sequencing.
Main Results:
- A Gram-positive, aerobic, non-motile, rod-shaped bacterium (strain P27(T)) was isolated.
- Optimal growth observed at 0-2.0% NaCl, pH 6-7, and 30°C.
- Unique chemotaxonomic profile including major menaquinones (MK-12, MK-11), fatty acids (anteiso-C17:0, anteiso-C15:0, iso-C16:0), cell-wall sugars (galactose, mannose, rhamnose), peptidoglycan (2,4-diaminobutyric acid, alanine, glutamic acid, glycine), and polar lipids (diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipid).
- Genomic DNA G+C content was 69.0 mol%.
- 16S rRNA gene sequence analysis showed highest similarity (97.0%) to Agromyces cerinus subsp. cerinus JCM 9083(T).
Conclusions:
- Strain P27(T) represents a novel species within the genus Agromyces.
- The proposed name for this new species is Agromyces atrinae sp. nov.
- The type strain is P27(T) (=KCTC 19593(T) =JCM 15913(T)).
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