Related Experiment Video

Updated: Jun 21, 2026

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
07:34

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Published on: April 16, 2019

Complicated urinary tract infection is associated with uroepithelial expression of proinflammatory protein S100A8

Leticia Reyes1, Sophie Alvarez, Ayman Allam

  • 1University of Florida, College of Veterinary Medicine, Department of Infectious Diseases & Pathology, P.O. Box 110880, Gainesville, FL 32611-0880, USA. lreyes@ufl.edu

Infection and Immunity
|August 12, 2009
PubMed

Insights

Invasive Ureaplasma parvum colonization triggers chronic inflammation and immune dysregulation, leading to complicated urinary tract infections (UTIs) and struvite urolithiasis in rats. Asymptomatic UTIs show limited inflammation.

Area of Science:

  • Microbiology
  • Immunology
  • Urology

Background:

  • Ureaplasma parvum infection in F344 rats can lead to either asymptomatic urinary tract infections (UTIs) or complicated UTIs with struvite urolithiasis.
  • Understanding the host immune response is crucial for differentiating disease outcomes.

Purpose of the Study:

  • To investigate the temporal host immune response to Ureaplasma parvum infection.
  • To identify host cellular responses distinguishing complicated UTIs from asymptomatic infections using differential quantitative proteomics.

Main Methods:

  • Histopathologic analysis and in situ localization of U. parvum.
  • Differential quantitative proteomics of bladder tissues.
  • Correlation analysis of protein and cytokine levels.

Main Results:

  • Persistent inflammation was observed in animals with invasive submucosal colonization, unlike resolved inflammation in asymptomatic cases.
  • Complicated UTIs showed increased proteins related to apoptosis, oxidative stress, and inflammation.
  • Elevated S100A8 protein in bladder tissue correlated with pro-inflammatory cytokines in urine, originating from the uroepithelium.

Conclusions:

  • Invasive Ureaplasma parvum colonization of the bladder triggers chronic inflammation and immune dysregulation.
  • This immune dysregulation is potentially critical for the development of struvite formation in complicated UTIs.

More Related Videos

Establishment and Characterization of UTI and CAUTI in a Mouse Model08:40

Establishment and Characterization of UTI and CAUTI in a Mouse Model

Published on: June 23, 2015

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization09:50

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

Published on: October 18, 2019

Related Concept Videos

Urinary Tract Infection II: Pathophysiology01:25

Urinary Tract Infection II: Pathophysiology

The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
Urinary Tract Infection I: Introduction01:26

Urinary Tract Infection I: Introduction

Urinary tract infections (UTIs) impact various parts of the urinary system, including the kidneys, ureters, bladder, and urethra. These infections are generally bacterial, with Escherichia coli being the most common causative agent, often originating from the gastrointestinal tract. However, other bacteria, such as Staphylococcus saprophyticus, Klebsiella pneumoniae, and Proteus mirabilis, are also known to cause UTIs. The type, location, and underlying complexity of the UTI guide both...
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care01:30

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
Acute Pyelonephritis I: Introduction01:27

Acute Pyelonephritis I: Introduction

Pyelonephritis is a bacterial infection that primarily affects the renal parenchyma and collecting system, including the renal pelvis, tubules, and interstitial tissue of one or both kidneys. It can be classified as either acute—a sudden, severe infection—or chronic, which refers to long-term or recurrent kidney infections.The primary cause of acute pyelonephritis (APN) is bacterial infection, with Escherichia coli accounting for approximately 70-80% of cases. Other bacteria, such as Proteus,...
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
Microbiota of the Urogenital Tract01:28

Microbiota of the Urogenital Tract

The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...