Nocardioides daedukensis sp. nov., a halotolerant bacterium isolated from soil

A Gram-positive, non-motile and rod- or coccoid-shaped bacterial strain, MDN22(T), was isolated from a soil sample from Korea. Strain MDN22(T) grew optimally at pH 7.0-8.0, at 30 degrees C and in the presence of 0-0.5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MDN22(T) was phylogenetically most closely related to the genera Nocardioides and Marmoricola. In the neighbour-joining phylogenetic tree, strain MDN22(T) was most closely related to Nocardioides jensenii KCTC 9134(T), with which it exhibited 98.3 % 16S rRNA gene sequence similarity. The strain exhibited 93.1-96.9 % and 95.3-95.9 % 16S rRNA gene sequence similarities to the type strains of other species of the genera Nocardioides and Marmoricola, respectively. The chemotaxonomic properties of strain MDN22(T) were consistent with those of the genus Nocardioides; the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acids were iso-C(16 : 0) and C(17 : 1). The DNA G+C content was 68.7 mol%. DNA-DNA relatedness data and differential phenotypic properties suggested that strain MDN22(T) could be differentiated from N. jensenii and Nocardioides dubius. On the basis of the data obtained, strain MDN22(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides daedukensis sp. nov., is proposed. The type strain is MDN22(T) (=KCTC 19601(T)=CCUG 57505(T)).