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Related Concept Videos

Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

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Related Experiment Video

Updated: Jun 21, 2026

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Distinct subcellular distribution of cyclin dependent kinase 6.

Dawn M Kohrt1, Jennifer I Crary, Vasilena Gocheva

  • 1Department of Biology, Connecticut College, New London, CT 06371, USA.

Cell Cycle (Georgetown, Tex.)
|August 12, 2009
PubMed
Summary

Cyclin-dependent kinase (CDK) 6, an oncogene, is found predominantly in the cytoplasm, not the nucleus, challenging its proposed roles in cell cycle regulation and differentiation.

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Related Experiment Videos

Last Updated: Jun 21, 2026

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

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Published on: May 3, 2018

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncogenes

Background:

  • Cyclin-dependent kinase (CDK) 6 is known to regulate the G1 phase of the cell cycle.
  • Emerging research suggests CDK6 also plays a role in cell differentiation.
  • Both proposed functions necessitate nuclear localization of CDK6.

Purpose of the Study:

  • To investigate the subcellular localization of CDK6.
  • To compare the localization of wild-type CDK6 with a common mutant form.
  • To assess if CDK6 localization aligns with its proposed nuclear functions.

Main Methods:

  • Analysis of CDK6 protein localization in cells.
  • Comparison between wild-type CDK6 and a mutant with a Y224D substitution.
  • Utilizing corrected wild-type sequence data from GenBank.

Main Results:

  • The majority of CDK6 protein is localized in the cytoplasm.
  • Significant concentrations of CDK6 are observed at the cell periphery and in cytoplasmic extensions.
  • The common mutant form was also analyzed for localization differences.

Conclusions:

  • CDK6's predominant cytoplasmic localization challenges its proposed nuclear roles in cell cycle control and differentiation.
  • Findings suggest a re-evaluation of CDK6's mechanisms in these cellular processes.
  • Understanding CDK6 localization is crucial for its role in cancer biology.