Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-nucleus brain transcriptomics reveals microglia dysfunction in multiple system atrophy.

Nature communications·2026
Same author

A population approach to cortical GABAergic interneuron function.

Neuron·2026
Same author

Defining the vascular niche of human adipose tissue across metabolic states.

Nature metabolism·2026
Same author

Male obesity causes adipose mitochondrial dysfunction in F<sub>1</sub> mouse progeny via a let-7-DICER axis.

Nature communications·2026
Same author

Oxytocin facilitates social behavior of female rats via selective modulation of interneurons in the medial prefrontal cortex.

Nature communications·2026
Same author

Heterogeneous generation and expansion of epidermal-resident memory T cells in individuals allergic to nickel.

The British journal of dermatology·2025

Related Experiment Video

Updated: Jun 21, 2026

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
11:32

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Published on: November 12, 2013

Major signaling pathways in migrating neuroblasts.

Konstantin Khodosevich1, Peter H Seeburg, Hannah Monyer

  • 1Department of Clinical Neurobiology, Interdisciplinary Center for Neurosciences Heidelberg, Germany.

Frontiers in Molecular Neuroscience
|August 12, 2009
PubMed
Summary

Researchers identified over 400 genes regulating neuronal migration in the rostral migratory stream (RMS). Specific gene networks, including cytoskeleton and calmodulin signaling, were confirmed to impact neuroblast movement to the olfactory bulb (OB).

Keywords:
RMS neuronal migrationSVZin vivo gene silencingmicroarray analysissignaling pathways

More Related Videos

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain
10:25

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

Published on: September 12, 2012

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
09:50

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Published on: April 20, 2018

Related Experiment Videos

Last Updated: Jun 21, 2026

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
11:32

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Published on: November 12, 2013

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain
10:25

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

Published on: September 12, 2012

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
09:50

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Published on: April 20, 2018

Area of Science:

  • Neuroscience
  • Developmental Biology

Background:

  • Neuronal migration is crucial for brain development and function.
  • The rostral migratory stream (RMS) is a significant postnatal neuronal migration pathway.
  • Understanding genes governing RMS neuroblast migration is key to comprehending brain wiring.

Purpose of the Study:

  • To identify novel genes and intracellular networks that regulate neuroblast migration within the RMS.
  • To validate the role of identified gene networks in directing neuroblasts to the olfactory bulb (OB).

Main Methods:

  • Isolation of RMS neuroblasts from transgenic mice expressing EGFP.
  • Microarray analysis to compare gene expression between different RMS locations.
  • Bioinformatic analysis to identify functional gene networks.
  • In vitro and in vivo functional assays to test gene network involvement in migration.

Main Results:

  • Over 400 upregulated genes were identified in RMS neuroblasts.
  • The cytoskeleton pathway was confirmed to significantly affect neuroblast migration.
  • Knockdown of genes in calmodulin signaling, Akt1-DNA transcription, and growth factor signaling networks impacted OB migration.

Conclusions:

  • This study identified numerous novel genes involved in RMS neuroblast migration.
  • Specific intracellular networks play critical roles in guiding neuroblasts to their destinations.
  • The findings provide new targets for understanding and potentially treating neurological disorders involving migration defects.