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Related Experiment Video

Updated: Jun 21, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

Pitfalls in TCR gene clonality testing: teaching cases.

Patricia J T A Groenen1, Anton W Langerak, Jacques J M van Dongen

  • 1Department of Pathology, Radboud University Nijmegen Medical Centre, Geert Grooteplein 24, 6525 GA, Nijmegen, The Netherlands, P.Groenen@pathol.umcn.nl.

Journal of Hematopathology
|August 12, 2009
PubMed
Summary

Clonality testing for T-cell receptor (TCR) gene analysis is now accessible. Accurate interpretation of TCR clonality data requires expertise and integration with other diagnostic methods for reliable T-lymphoproliferation diagnosis.

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Area of Science:

  • Hematology
  • Molecular Biology
  • Immunology

Background:

  • Clonality testing in T-lymphoproliferations is crucial for diagnosis.
  • Standardized multiplex polymerase chain reaction (PCR) protocols for T-cell receptor (TCR) gene analysis are now available.
  • BIOMED-2 Concerted Action BMH4-CT98-3936 developed these standardized protocols.

Purpose of the Study:

  • To address potential pitfalls in interpreting TCR clonality data.
  • To emphasize the importance of expertise in clonality diagnostics and TCR gene biology.
  • To advocate for an integrated diagnostic approach.

Main Methods:

  • Utilizing standardized multiplex PCR protocols for TCR gene analysis.
  • Analyzing potential immunobiological and technical pitfalls.

Related Experiment Videos

Last Updated: Jun 21, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
11:21

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Published on: September 10, 2017

  • Integrating molecular data with immunohistology and flow cytometry.

    • Main Results:

    • Standardized TCR gene analysis protocols facilitate routine clonality testing.
    • Several pitfalls can lead to misinterpretation of TCR clonality data.
    • Multidisciplinary integration of diagnostic data is essential.

    Conclusions:

    • Expertise and awareness of pitfalls are vital for accurate TCR clonality data interpretation.
    • Integrating molecular data with immunohistology and flow cytometry improves diagnostic reliability.
    • A multidisciplinary diagnostic model ensures the most accurate diagnosis of T-lymphoproliferations.