Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Soluble CD8 antigen in systemic sclerosis.

G Famularo1, R Giacomelli, S Sacchetti

  • 1Dipartimento di Medicina Interna e Sanità Pubblica, Università degli Studi di L'Aquila, Italy.

Journal of Clinical & Laboratory Immunology
|July 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Upadacitinib regulates pain-related pathways and BDNF expression in human monocyte-derived microglial-like cells.

Brain, behavior, and immunity·2025
Same author

Probable apixaban-induced purpura.

European annals of allergy and clinical immunology·2023
Same author

In Vitro Trials: The Dawn of a New Era for Drug Discovery in Atopic Dermatitis?

The Journal of pharmacology and experimental therapeutics·2023
Same author

Endothelial-to-mesenchymal transition in systemic sclerosis.

Clinical and experimental immunology·2021
Same author

Clinical features and prognosis of COVID-19 in people with spinal cord injury: a case-control study.

Spinal cord series and cases·2020
Same author

Evaluation of the influence of social, demographic, environmental, work-related factors and/or lifestyle habits on Raynaud's phenomenon: a case-control study.

Clinical and experimental medicine·2019
Same journal

Policy and science of FMD control: the stakeholders' contribution to decision making. A call for integrated animal disease management.

Journal of clinical & laboratory immunology·2006
Same journal

An informative case of Graves' disease with implications for schizophrenia.

Journal of clinical & laboratory immunology·2006
Same journal

Increase of circulating CD8+CD57+ lymphocytes after measles infection but not after measles vaccination.

Journal of clinical & laboratory immunology·2006
Same journal

Review: immunomodulatory activity of pregnancy-associated plasma protein-A.

Journal of clinical & laboratory immunology·2004
Same journal

Antibodies to Acinetobacter bacteria and bovine brain peptides, measured in bovine spongiform encephalopathy (BSE) in an attempt to develop an ante-mortem test.

Journal of clinical & laboratory immunology·2004
Same journal

Principles of autoimmune disease: pathogenesis, genetics and specific immunotherapy.

Journal of clinical & laboratory immunology·2004
See all related articles

This study on systemic sclerosis (PSS) found that CD8+ cell activation is not a major issue. Soluble CD8 antigen levels and activated CD8+ cell frequency were similar in PSS patients and healthy controls.

Area of Science:

  • Immunology
  • Rheumatology
  • Cell Biology

Background:

  • Systemic sclerosis (PSS) is an autoimmune disease characterized by fibrosis and vascular abnormalities.
  • The role of CD8+ lymphocytes in the pathogenesis of PSS remains incompletely understood.
  • Assessing CD8+ T-cell function may provide insights into PSS immune dysregulation.

Purpose of the Study:

  • To evaluate the function of circulating CD8+ lymphocytes in patients with PSS.
  • To determine levels of soluble CD8 antigen (sCD8) and the frequency of activated CD8+ cells in PSS patients.

Main Methods:

  • Quantified soluble CD8 antigen (sCD8) in serum and peripheral blood mononuclear cell (PBMC) culture supernatants from 15 PSS patients and controls.
  • Utilized cytofluorographic analysis to determine the frequency of activated CD8+ cells expressing DR antigens, IL-2 receptor, and transferrin receptor.

Related Experiment Videos

Main Results:

  • Elevated sCD8 levels were detected in only 6 out of 15 PSS patients compared to healthy controls.
  • Slightly increased sCD8 was observed in PBMC culture media from PSS patients.
  • The frequency of circulating activated CD8+ cells was comparable between PSS patients and controls.

Conclusions:

  • CD8+ T-cell activation does not appear to be a significant immunological feature in systemic sclerosis.
  • Further research is needed to fully elucidate the immune mechanisms underlying PSS pathogenesis.