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Super-resolution imaging with small organic fluorophores

Mike Heilemann1, Sebastian van de Linde, Anindita Mukherjee

  • 1Applied Laser Physics & Laser Spectroscopy, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany. heileman@physik.uni-bielefeld.de

Angewandte Chemie (International Ed. in English)
|August 12, 2009
PubMed
Summary

No abstract available in PubMed .

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Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.