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Related Experiment Video

Updated: Jun 21, 2026

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Published on: November 16, 2015

[Immunologic function of palatine tonsil B lymphocyte].

Mike Min¹, Chaowu Ma, Boquan Jin

¹Department of Otolaryngology, No. 117 Hospital of PLA, Hangzhou, 310004, China. meek@263.net

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Journal of Clinical Otorhinolaryngology Head and Neck Surgery

|August 13, 2009

Summary

Palatine tonsil B cells show higher CD20 expression than peripheral blood cells, but their cellular immune function remains similar. Further research is needed to understand the implications of this difference.

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Last Updated: Jun 21, 2026

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Published on: November 16, 2015

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

Published on: June 14, 2020

Area of Science:

Immunology
Cell Biology

Background:

B cells play a crucial role in the adaptive immune system.
Palatine tonsils are key sites for initiating immune responses.
Understanding B cell function in tonsils is vital for immune health.

Purpose of the Study:

To investigate the cellular immune function of B lymphocytes in palatine tonsils.
To compare the phenotype and function of palatine tonsil B cells with peripheral blood B cells.

Main Methods:

Phenotypic analysis of palatine tonsil cells (PTC) and peripheral blood mononuclear cells (PBMC) using flow cytometry.
Isolation of CD3- cells from PTC and PBMC using immunomagnetic beads.
Assessment of B cell proliferation following stimulation with CD20 monoclonal antibody (mAb).

Main Results:

Flow cytometry revealed significantly higher CD20 expression on PTC (71.2%) compared to PBMC (15.5%).
Mean fluorescence intensity (MFI) for CD20 was higher in palatine tonsil B cells.
No significant difference was observed in the proliferation capacity between palatine tonsil and peripheral blood B cells.

Conclusions:

CD20 expression levels differ notably between palatine tonsil and peripheral blood B cells.
The functional consequences of differential CD20 expression on B cell immunity are yet to be elucidated.
Further studies are warranted to explore the functional implications of these findings.