Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methylated circulating tumor DNA: technical challenges and clinical applications in non-small cell lung cancer patients-a narrative review.

Translational lung cancer research·2026
Same author

Mapping of CELF1-RNA interactions reveals post-transcriptional control of lens development.

bioRxiv : the preprint server for biology·2026
Same author

Insights into the evolution and regulation of hybrid internal-terminal exons from tropomyosin exon 9A in Xenopus laevis.

Biochimie·2025
Same author

Transcriptome Meta-Analysis Uncovers Cell-Specific Regulatory Relationships in Embryonic, Juvenile, Adult, and Aged Mouse Lens Epithelium and Fibers.

Investigative ophthalmology & visual science·2025
Same author

Absolute Quantification of Nucleotide Variants in Cell-Free DNA via Quantitative NGS: Clinical Application in Non-Small Cell Lung Cancer Patients.

Cancers·2025
Same author

Proteomic study identifies Aurora-A-mediated regulation of alternative splicing through multiple splicing factors.

The Journal of biological chemistry·2024

Related Experiment Video

Updated: Jun 21, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Analysis of splicing patterns by pyrosequencing.

Agnès Méreau1, Vincent Anquetil, Marie Cibois

  • 1Institut de Génétique et Développement de Rennes, Université de Rennes 1, IFR 140, CNRS, UMR6061, Equipe Expression Génétique et Développement, Université Européenne de Bretagne, F-35000 Rennes, France.

Nucleic Acids Research
|August 13, 2009
PubMed
Summary

This study introduces Pyrosequencing Analysis of Splicing Patterns (PASP), a reliable method for quantifying RNA splicing patterns. PASP enables high-throughput analysis of alternative splicing events crucial for understanding development and disease.

More Related Videos

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Related Experiment Videos

Last Updated: Jun 21, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Alternative splicing generates diverse mRNA isoforms from a single pre-mRNA.
  • Splicing pattern analysis is vital for understanding gene regulation, cellular processes, and diseases.

Purpose of the Study:

  • To introduce and validate a novel method, Pyrosequencing Analysis of Splicing Patterns (PASP), for analyzing RNA splicing patterns.
  • To demonstrate the adaptability and reliability of PASP for various splicing events and biological samples.

Main Methods:

  • RT-PCR combined with pyrosequencing of PCR products.
  • Method validation using mixed RNA samples and comparison with existing techniques.
  • Adaptation for diverse splicing events including exon skipping, inclusion, and alternative 3' terminal exons.

Main Results:

  • PASP accurately quantifies RNA ratios across various proportions.
  • The method successfully analyzes complex splicing patterns like mutually exclusive exons.
  • PASP measurements correlate well with other established RNA analysis methods across different organs.

Conclusions:

  • PASP is a reliable and versatile method for analyzing splicing patterns.
  • The high-throughput, gel-free nature of PASP facilitates large-scale RNA comparisons.
  • PASP offers a valuable tool for research in developmental biology, cellular responses, and human diseases.