Related Experiment Videos
A novel homodimeric molecule involved in human T cell activation
T Kobata1, H Yagita, H Matsuda
1Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1990
Summary
A novel monoclonal antibody (mAb), 10D1, activates human CD4+ T cells through a pathway linked to the CD3/TCR complex. This discovery offers new insights into T cell activation mechanisms.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Monoclonal antibodies (mAbs) are crucial tools in immunology research.
- Understanding T cell activation pathways is key to developing immunotherapies.
Purpose of the Study:
- To characterize a novel monoclonal antibody, 10D1, targeting human T cells.
- To investigate the role of the 10D1 molecule in T cell activation.
Main Methods:
- Hybridoma technology for mAb production.
- Flow cytometry for cell surface marker analysis.
- Cell proliferation assays and intracellular calcium flux measurements.
Main Results:
- 10D1 mAb specifically recognizes T cells, particularly CD4+ T cells.
- 10D1 induces proliferation of peripheral blood T cells, dependent on CD4+ cells.
- 10D1-induced activation is associated with the CD3/TCR complex and increases intracellular calcium.
Conclusions:
- The 10D1 molecule is involved in a novel pathway of human CD4+ T cell activation.
- This pathway is linked to the CD3/TCR-mediated signaling cascade.
- 10D1 provides a new tool for studying T cell activation.