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Related Experiment Video

Updated: Jun 21, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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New approach for m-cell-specific molecules screening by comprehensive transcriptome analysis.

Gaku Nakato1, Shinji Fukuda, Koji Hase

  • 1International Graduate School of Arts and Sciences, Yokohama City University, Kanagawa 230-0045, Japan.

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
|August 14, 2009
PubMed
Summary
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Researchers identified new M cell molecules by comparing gene expression in mice and chickens. These findings reveal conserved M cell proteins important for immune function and differentiation in both species.

Area of Science:

  • Immunology
  • Cell Biology
  • Genomics

Background:

  • M cells in Peyer's patches (PPs) are crucial for antigen uptake but rare in mammals.
  • Avian M cells are abundant, offering a model for comparative study.
  • Identifying M cell-specific molecules is key to understanding their function.

Purpose of the Study:

  • To identify conserved M cell-specific genes and molecules in avian and mammalian species.
  • To characterize novel M cell surface proteins involved in antigen sampling.
  • To understand the functional and differentiation roles of these conserved molecules.

Main Methods:

  • Comparative gene expression profiling of chicken and murine follicle-associated epithelium (FAE).
  • In situ hybridization to localize gene expression.

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Last Updated: Jun 21, 2026

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Published on: October 25, 2018

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  • Whole-mount immunostaining to identify protein localization and co-expression.

    • Main Results:

    • 28 genes were commonly up-regulated in FAE from both chickens and mice.
    • Annexin A10 (Anxa10) mRNA and cellular prion protein (PrP(C)) were identified as M cell-specific markers.
    • PrP(C) co-localized with glycoprotein 2 on M cell surfaces.

    Conclusions:

    • Comparative transcriptomics successfully identified conserved M cell molecules.
    • Annexin A10 and PrP(C) are novel, species-conserved M cell markers.
    • These conserved molecules likely play vital roles in M cell function and differentiation.