Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noninvasive Detection of ctDNA Reveals Intratumor Heterogeneity and Is Associated with Tumor Burden in Gastrointestinal Stromal Tumor.

Molecular cancer therapeutics·2018
Same author

ALK Is a Specific Diagnostic Marker for Inflammatory Myofibroblastic Tumor of the Uterus.

The American journal of surgical pathology·2018
Same author

Molecular Profiling of Hyalinizing Clear Cell Carcinomas Revealed a Subset of Tumors Harboring a Novel EWSR1-CREM Fusion: Report of 3 Cases.

The American journal of surgical pathology·2018
Same author

Uterine Inflammatory Myofibroblastic Tumors Frequently Harbor ALK Fusions With IGFBP5 and THBS1.

The American journal of surgical pathology·2017
Same author

Detection of Novel t(12;17)(p12;p13) in Relapsed Refractory Acute Myeloid Leukemia by Anchored Multiplex PCR(AMP)-based Next-Generation Sequencing.

Applied immunohistochemistry & molecular morphology : AIMM·2017
Same author

The application of next-generation sequencing-based molecular diagnostics in endometrial stromal sarcoma.

Histopathology·2016

Related Experiment Video

Updated: Jun 21, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Systematic analysis of dynamic miRNA-target interactions during C. elegans development.

Liang Zhang1, Molly Hammell, Brian A Kudlow

  • 1Howard Hughes Medical Institute and Department of MCDB, University of Colorado, Boulder, CO 80309, USA.

Development (Cambridge, England)
|August 14, 2009
PubMed
Summary

Researchers systematically analyzed microRNA (miRNA) and mRNA interactions during C. elegans development. They identified stage-specific miRNAs and thousands of their targets, revealing principles of miRNA regulation in development.

More Related Videos

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
10:58

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Published on: February 27, 2012

Related Experiment Videos

Last Updated: Jun 21, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
10:58

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Published on: February 27, 2012

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play crucial roles in animal development, but most miRNA::mRNA regulatory interactions are not experimentally characterized.
  • Understanding these interactions is essential for deciphering developmental programs.
  • Systematic analysis of miRNA::mRNA interactions across developmental stages is needed.

Purpose of the Study:

  • To systematically analyze miRNA::mRNA interactions during C. elegans development using a novel immunoprecipitation approach.
  • To characterize stage-specific miRNA functions and identify their mRNA targets.
  • To elucidate the principles of physiological miRNA::target recognition and predict high-confidence interactions.

Main Methods:

  • Utilized an immunoprecipitation approach targeting AIN/GW182 proteins to isolate functional miRNA-induced silencing complexes (miRISCs).
  • Analyzed miRNA composition within miRISCs at different developmental stages of C. elegans.
  • Identified thousands of miRNA targets and miRNA family-mRNA pairs with temporally correlated association patterns.

Main Results:

  • Identified three sets of miRNAs with distinct stage-specific functions during C. elegans development.
  • Characterized thousands of miRNA targets per developmental stage, with many showing differential regulation.
  • Predicted 1589 high-confidence miRNA family::mRNA interactions based on temporal association patterns.

Conclusions:

  • miRNAs preferentially target genes involved in signaling processes and avoid housekeeping genes during development.
  • miRNAs orchestrate temporal developmental programs by coordinately targeting or avoiding specific biological functions.
  • The study provides valuable insights into the principles of miRNA::target recognition in a developmental context.