Related Experiment Video
Updated: Jun 21, 2026

Determining Basal Energy Expenditure and the Capacity of Thermogenic Adipocytes to Expend Energy in Obese Mice
Published on: November 11, 2021
Measurement of resting energy expenditure in healthy children
Robin R Mellecker1, Alison M McManus
1Institute of Human Performance, University of Hong Kong, Hong Kong, China. robinmel@hkusua.hku.hk
Insights
A 20-minute resting energy expenditure (REE) measurement using a face mask is recommended for children. This method offers greater accuracy and comfort, improving compliance for pediatric obesity research.
Area of Science:
- Pediatric physiology
- Metabolic research
- Obesity studies
Background:
- Childhood obesity is a growing concern, necessitating accurate measurement of resting energy expenditure (REE).
- Current REE measurement protocols for children lack standardization, leading to variability in results.
- This study addresses the need for reliable REE assessment methods in pediatric populations.
Purpose of the Study:
- To evaluate the impact of measurement duration on REE in children.
- To compare the effectiveness of a mask versus a mouthpiece/noseclip for REE measurement.
- To identify the optimal protocol for accurate and comfortable REE assessment in children.
Main Methods:
- Twenty-three healthy children (7-12 years) underwent two 35-minute indirect calorimetry protocols after a 12-hour fast.
- Measurements were taken using either a face mask or a mouthpiece/noseclip.
- Device comfort was rated on a 6-point scale.
Main Results:
- No significant differences in REE were found between 10, 15, 20, 25, and 30 minutes of rest for either device.
- The mask showed the least variation in energy expenditure (6% CV) after 20 minutes.
- Children reported significantly less discomfort with the mask compared to the mouthpiece/noseclip.
Conclusions:
- A 20-minute REE measurement protocol using a face mask is suggested for children.
- This protocol may enhance patient compliance and practicality in pediatric research.
- Standardizing REE measurement is crucial for advancing childhood obesity research.
Background:
The role that the components of energy expenditure play in the etiology of childhood obesity has highlighted the need for greater accuracy and standardized protocols for the measurement of resting energy expenditure (REE). However, protocols used to assess REE in children are varied, and consensus on a suitable method for measuring REE in children has not been reached. This study was undertaken to determine the effect of measurement time and measurement device (mask or mouthpiece) on REE in healthy children.
Design:
Following a 12-hour fast and abstinence from exercise, 23 children (age, 7-12 years) completed two 35-minute protocols: one with a face mask and the other with a mouthpiece/noseclip. Energy expenditure was measured continuously via indirect calorimetry, while device acceptability was assessed using a 6-point comfort rating scale.
Results:
Repeated measures ANOVA indicated that there was no significant difference in REE when measured after 10, 15, 20, or 25 minutes of rest compared to 30 minutes for either the mask or mouthpiece/noseclip (REE range, 1371-1460 kcal/d). Examination of the percentage coefficient of varia tion (CV) in energy expenditure for each time period by device showed that the least variation existed after 20 minutes of measurement using the mask (CV 6%). Paired t test analysis indicated significantly less discomfort when wearing the mask compared to the mouthpiece/noseclip.
Conclusion:
It would appear that a 20-minute protocol using a mask may increase compliance and prove to be a more practical protocol for measuring REE in children.
Related Concept Videos
Metabolic Rate
The Basal Metabolic Rate (BMR) measures the energy expended at rest.
Several factors influence the...
Energy Balance
Energy Budgets and Reproductive Strategies

