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Updated: Jun 21, 2026

A Protocol for Computer-Based Protein Structure and Function Prediction
Published on: November 3, 2011
SubChlo: predicting protein subchloroplast locations with pseudo-amino acid composition and the evidence-theoretic
Pufeng Du1, Shengjiao Cao, Yanda Li
1MOE Key Laboratory of Bioinformatics and Bioinformatics Division, TNLIST/Department of Automation, Tsinghua University, Beijing 100084, China. dpf05@mails.tsinghua.edu.cn
Abstract:
The chloroplast is a type of plant specific subcellular organelle. It is of central importance in several biological processes like photosynthesis and amino acid biosynthesis. Thus, understanding the function of chloroplast proteins is of significant value. Since the function of chloroplast proteins correlates with their subchloroplast locations, the knowledge of their subchloroplast locations can be very helpful in understanding their role in the biological processes. In the current paper, by introducing the evidence-theoretic K-nearest neighbor (ET-KNN) algorithm, we developed a method for predicting the protein subchloroplast locations. This is the first algorithm for predicting the protein subchloroplast locations. We have implemented our algorithm as an online service, SubChlo (http://bioinfo.au.tsinghua.edu.cn/subchlo). This service may be useful to the chloroplast proteome research.
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