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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
A rapid allele variant discrimination method for Yersinia pestis strains based on high-resolution melting curve
Andrea Ciammaruconi1, Saverio Grassi, Giovanni Faggioni
1Army Medical Research Center, 00184 Rome, Italy. andrea.ciammaruconi@gmail.com
Abstract:
Yersinia pestis isolates were genotyped analyzing the polymorphic DNA regions named variable number tandem repeats (VNTR). Allele variants were studied by high-resolution melting analysis (HRMA) of polymerase chain reaction fragments obtained for 25 VNTR loci. After comparison with previous results, 14 loci gave distinguishable normalized melting curves and allowed to correctly assign alleles. This HRMA typing technique permits to differentiate Y. pestis isolates and turned out to be robust, reproducible, and cheap.
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