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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
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Published on: January 27, 2012

Monitoring protein kinases in cellular media with highly selective chimeric reporters

Elvedin Luković1, Elizabeth Vogel Taylor, Barbara Imperiali

  • 1Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA, USA.

Angewandte Chemie (International Ed. in English)
|August 15, 2009
PubMed
Abstract

No abstract available in PubMed .

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...